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Publication Detail

Title: Adaptation of a gamma-glutamyl transpeptidase assay to microtiter plates.

Authors: Silber, P M; Gandolfi, A J; Brendel, K

Published In Anal Biochem, (1986 Oct)

Abstract: A kinetic assay for measuring gamma-glutamyl transpeptidase (GGT) activity has been adapted to microtiter plates and an automated microtiter plate reader. This method permits the simultaneous analysis of enzyme activity in a large number of samples incubated with the chromogenic GGT substrate gamma-glutamyl-p-nitroanilide. A major advantage of this assay over previously reported methods is the substantial reduction in the time needed for measuring sample enzyme activity. In addition, reduction of the total assay volume to 0.28 ml conserves both sample and reagents. This method has been calibrated at 23 degrees C using purified GGT, and used to analyze GGT activity in human sera. The assay is sensitive over a range of 3-200 U/liter.

PubMed ID: 2879485 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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