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Title: Nonredundant functions of alphabeta and gammadelta T cells in acrolein-induced pulmonary pathology.

Authors: Borchers, Michael T; Wesselkamper, Scott C; Eppert, Bryan L; Motz, Gregory T; Sartor, Maureen A; Tomlinson, Craig R; Medvedovic, Mario; Tichelaar, Jay W

Published In Toxicol Sci, (2008 Sep)

Abstract: Acrolein exposure represents a significant human health hazard. Repeated acrolein exposure causes the accumulation of monocytes/macrophages and lymphocytes, mucous cell metaplasia, and epithelial injury. Currently, the mechanisms that control these events are unclear, and the relative contribution of T-cell subsets to pulmonary pathologies following repeated exposures to irritants is unknown. To examine whether lymphocyte subpopulations regulate inflammation and epithelial cell pathology, we utilized a mouse model of pulmonary pathology induced by repeated acrolein exposures. The role of lymphocyte subsets was examined by utilizing transgenic mice genetically deficient in either alphabeta T cells or gammadelta T cells, and changes in cellular, molecular, and pathologic outcomes associated with repeated inhalation exposure to 2.0 and 0.5 ppm acrolein were measured. To examine the potential functions of lymphocyte subsets, we purified these cells from the lungs of mice repeatedly exposed to 2.0 ppm acrolein, isolated and amplified messenger RNA, and performed microarray analysis. Our data demonstrate that alphabeta T cells are required for macrophage accumulation, whereas gammadelta T cells are critical regulators of epithelial cell homeostasis, as identified by epithelial cell injury and apoptosis, following repeated acrolein exposure. This is supported by microarray analyses that indicated the T-cell subsets are unique in their gene expression profiles following acrolein exposures. Microarray analyses identified several genes that may contribute to phenotypes mediated by T-cell subpopulations including those involved in cytokine receptor signaling, chemotaxis, growth factor production, lymphocyte activation, and apoptosis. These data provide strong evidence that T-cell subpopulations in the lung are major determinants of pulmonary pathology and highlight the advantages of dissecting their effector functions in response to toxicant exposures.

PubMed ID: 18515264 Exiting the NIEHS site

MeSH Terms: Acrolein/toxicity*; Animals; Apoptosis/drug effects; Cell Separation; Epithelial Cells/drug effects; Epithelial Cells/pathology; Gene Expression Profiling; Interleukin-18/physiology; Lung/drug effects*; Lung/immunology; Lung/pathology; Macrophages/drug effects; Macrophages/physiology; Mice; Mice, Inbred C57BL; Mucin 5AC; Mucins/analysis; Oligonucleotide Array Sequence Analysis; Receptors, Antigen, T-Cell, alpha-beta/physiology*; Receptors, Antigen, T-Cell, gamma-delta/physiology*; T-Lymphocytes/physiology*

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Last Reviewed: October 07, 2024