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National Institute of Environmental Health Sciences

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Publication Detail

Title: Accurate quantitation of standard peptides used for quantitative proteomics.

Authors: Bordeerat, Narisa K; Georgieva, Nadia I; Klapper, David G; Collins, Leonard B; Cross, Tyra J; Borchers, Christoph H; Swenberg, James A; Boysen, Gunnar

Published In Proteomics, (2009 Aug)

Abstract: MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.

PubMed ID: 19637239 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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