Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Identification of the major protein adduct formed in rat liver after thioacetamide administration.

Authors: Dyroff, M C; Neal, R A

Published In Cancer Res, (1981 Sep)

Abstract: The in vivo covalent binding of the hepatocarcinogen thioacetamide to rat liver protein has been examined. Following administration of 3H- or 14C-labeled thioacetamide, the modified amino acids present in the hepatic cytosolic proteins were isolated by enzymatic digestion and ion-exchange chromatography. Approximately 70% of the radioactivity covalently bound to cytosolic protein was recovered in a compound which upon acid hydrolysis yielded lysine and radiolabeled acetate. Additional studies indicated the structure of this adduct was N-epsilon-acetyllysine.

PubMed ID: 6790164 Exiting the NIEHS site

MeSH Terms: Acetamides/administration & dosage*; Amino Acids/analysis; Animals; Chromatography, Ion Exchange; Gas Chromatography-Mass Spectrometry; Liver/drug effects*; Liver/metabolism; Lysine/analogs & derivatives; Lysine/analysis; Male; Proteins/analysis; Proteins/isolation & purification*; Rats; Thioacetamide/administration & dosage*

Back
to Top