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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Microsomal bioactivation and covalent binding of aliphatic halides to DNA.

Authors: DiRenzo, A B; Gandolfi, A J; Sipes, I G

Published In Toxicol Lett, (1982 May)

Abstract: Studies were carried out on the in vitro covalent binding of a series of 14C-labeled aliphatic halides to calf thymus DNA following bioactivation by hepatic microsomes isolated from phenobarbital-treated rats. Six compounds were shown to exhibit binding to DNA of greater than 0.3 nmol/mg DNA (1,2-dibromoethane, bromotrichloromethane, trichloroethylene, carbon tetrachloride, chloroform, and 1,1,2-trichloroethane). Covalent binding of the aliphatic halides to the nucleic acids was confirmed by sedimentation of the DNA-organohalogen adduct in a cesium chloride gradient and Sephadex LH-20 chromatography of the nucleosides released by enzymatic hydrolysis.

PubMed ID: 7101318 Exiting the NIEHS site

MeSH Terms: Animals; Biotransformation; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry; Chromatography; DNA/metabolism; Hydrocarbons, Halogenated/metabolism*; Liver/enzymology; Microsomes, Liver/metabolism*; Rats

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