Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Effect of growth rate on phenotypic expression of 6-thioguanine resistance in human diploid fibroblasts.

Authors: Grosovsky, A J; Little, J B

Published In Mutat Res, (1983 Jun-Jul)

Abstract: The influence of growth rate on the kinetics of phenotypic expression of 6-thioguanine resistance has been investigated by establishing 3 protocols for the maintenance of mutagenized stock cultures of human diploid fibroblasts. These protocols differed in the frequency with which subculture was performed during the phenotypic expression period. The resultant growth kinetics varied widely from continual exponential proliferation to density-inhibited growth for much of the expression period. With all of the subculture protocols, a maximum mutation frequency was reached by 9 days after exposure of confluent cultures to 6 J/m2 of 254 nm UV light. The maximum frequency was similar in all experimental groups and remained stable up to 17 days after irradiation, the last time point tested. These results indicate that continual proliferation is not required for optimal expression of 6-thioguanine resistance.

PubMed ID: 6865995 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

Back
to Top