Title: Characterization of a quantitative assay for the in vitro transformation of normal human diploid fibroblasts to anchorage independence by chemical carcinogens.

Authors: Zimmerman, R J; Little, J B

Published In Cancer Res, (1983 May)

Abstract: We have characterized an assay for the quantitative measurement of the frequency of conversion to anchorage-independent growth of N-acetoxy-2-acetylaminofluorene-treated normal human diploid fibroblasts. We investigated the effects of the following parameters on the absolute number and on the frequency of anchorage-independent colonies scored: (a) the number of cells seeded per dish; (b) the type of posttreatment medium; (c) the number of population doublings allowed posttreatment prior to seeding in suspension; and (d) the carcinogen dose. The assay was linear over the range of 1.9 X 10(3) to 3.8 X 10(4) cells seeded per 6-mm dish for both total colonies scored and the induced frequency of anchorage-independent growth. The medium used posttreatment affected both the frequency and the kinetics of appearance of the anchorage-independent phenotype. The number of population doublings and the number of days allowed posttreatment prior to assaying for anchorage-independent growth potential also influenced the frequency of recovery of this phenotype. Under standardized conditions, the assay yielded a dose-response relationship for transformation to anchorage independence over the concentration range of 0 to 10 microM N-acetoxy-2-acetylaminofluorene.

PubMed ID: 6339048

MeSH Terms: No MeSH terms associated with this publication