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Publication Detail

Title: Comparison of prometaphase chromosome techniques with emphasis on the role of colcemid.

Authors: Wiley, J E; Sargent, L M; Inhorn, S L; Meisner, L F

Published In In Vitro, (1984 Dec)

Abstract: Six different techniques were evaluated to define better those technical factors that are most critical for obtaining prometaphase cells for banding analysis. Our results demonstrate: colcemid exposures of 30 min or less have no effect on increasing the yield of prometaphase cells, colcemid exposures of greater than 0.1 microgram/ml can be toxic, methotrexate depresses the mitotic index significantly and seems to increase the incidence of prometaphase cells only because it suppresses later forms; and (d) the optimum number of cytogenetically satisfactory prometaphase cells can be obtained with a 4-h exposure to a combination of low concentration actinomycin D (0.5 microgram/ml) and colcemid (0.1 microgram/ml). This technique inhibits chromosome condensation while permitting prometaphase cells to accumulate for 4 h.

PubMed ID: 6530228 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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