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Title: Chronic cigarette smoke exposure primes NK cell activation in a mouse model of chronic obstructive pulmonary disease.

Authors: Motz, Gregory T; Eppert, Bryan L; Wortham, Brian W; Amos-Kroohs, Robyn M; Flury, Jennifer L; Wesselkamper, Scott C; Borchers, Michael T

Published In J Immunol, (2010 Apr 15)

Abstract: Chronic obstructive pulmonary disease (COPD) is a debilitating, progressive lung disease punctuated by exacerbations of symptoms. COPD exacerbations are most often associated with viral infections, and exposure to cigarette smoke (CS) followed by viral infection has been shown experimentally to enhance lung inflammation, tissue destruction, and airway fibrosis. Despite this, however, the cellular mechanisms responsible for this effect are unknown. In this study, we examined NK cell function in a mouse model of COPD given the vital role of NK cells following viral infection. Ex vivo stimulation of lung leukocytes with poly(I:C), ssRNA40, or ODN1826 enhanced production of NK cell-derived IFN-gamma in CS-exposed mice. NK cells from CS-exposed mice exhibited a novel form of priming; highly purified NK cells from CS-exposed mice, relative to NK cells from filtered air-exposed mice, produced more IFN-gamma following stimulation with IL-12, IL-18, or both. Further, NK cell priming was lost following smoking cessation. NKG2D stimulation through overexpression of Raet1 on the lung epithelium primed NK cell responsiveness to poly(I:C), ssRNA40, or ODN1826 stimulation, but not cytokine stimulation. In addition, NK cells from CS-exposed mice expressed more cell surface CD107a upon stimulation, demonstrating that the NK cell degranulation response was also primed. Together, these results reveal a novel mechanism of activation of the innate immune system and highlight NK cells as important cellular targets in controlling COPD exacerbations.

PubMed ID: 20228194 Exiting the NIEHS site

MeSH Terms: Animals; Cells, Cultured; Coculture Techniques; DNA/toxicity; Disease Models, Animal; Female; Inflammation Mediators/pharmacology; Inflammation Mediators/toxicity*; Interferon-gamma/biosynthesis; Killer Cells, Natural/immunology*; Killer Cells, Natural/metabolism*; Killer Cells, Natural/virology; Lung/cytology; Lung/immunology; Lung/metabolism; Lymphocyte Activation/immunology*; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Oligodeoxyribonucleotides; Poly I-C/toxicity; Pulmonary Disease, Chronic Obstructive/immunology*; Pulmonary Disease, Chronic Obstructive/pathology; Pulmonary Disease, Chronic Obstructive/virology; RNA, Viral/toxicity; Tobacco Smoke Pollution/adverse effects*; Up-Regulation/immunology

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