Title: Conserved tyrosine in the first transmembrane segment of solute:sodium symporters is involved in Na+-coupled substrate co-transport.

Authors: Mazier, Sonia; Quick, Matthias; Shi, Lei

Published In J Biol Chem, (2011 Aug 19)

Abstract: Solute:sodium symporters (SSSs) transport vital molecules across the plasma membrane of all living organisms. vSGLT, the Na(+)/galactose transporter of Vibrio parahemeolyticus, is the only SSS for which high resolution structural information is available, revealing a LeuT-like fold and a Na(+)-binding site analogous to the Na2 site of LeuT. Whereas the core transmembrane segments (TMs) of SSSs share high structural similarity with other transporters of LeuT-like fold, TM1 does not correspond to any TM in those structural homologs and was only resolved for the backbone atoms in the initial vSGLT structure (Protein Data Bank code 3DH4). To assess the role of TM1 in Na(+)-coupled substrate symport by the SSSs, here we have studied the role of a conserved residue in TM1 by computational modeling in conjunction with radiotracer transport and binding studies. Based on our sequence alignment and much topological data for homologous PutP, the Na(+)/proline transporter, we have simulated a series of vSGLT models with shifted TM1 residue assignments. We show that in two converged vSGLT models that retained the original TM1 backbone conformation, a conserved residue, Tyr-19, is associated with the Na(+) binding interaction network. In silico and in vitro mutagenesis of homologous Tyr-14 in PutP revealed the involvement of this conserved residue in Na(+)-dependent substrate binding and transport. Thus, our combined computational and experimental data provide the first clues about the importance of a conserved residue in TM1, a unique TM in the proteins with LeuT-like fold, in the Na(+)-coupled symport mechanism of SSSs.

PubMed ID: 21705334

MeSH Terms: Amino Acid Motifs; Bacterial Proteins/chemistry*; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport/physiology; Databases, Protein; Galactose/chemistry*; Galactose/metabolism; Models, Molecular*; Protein Folding*; Protein Structure, Tertiary; Sodium/chemistry*; Sodium/metabolism; Structure-Activity Relationship; Symporters/chemistry*; Symporters/genetics; Symporters/metabolism; Vibrio parahaemolyticus/chemistry*; Vibrio parahaemolyticus/genetics; Vibrio parahaemolyticus/metabolism