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National Institute of Environmental Health Sciences

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Publication Detail

Title: Kit-On-A-Lid-Assays for accessible self-contained cell assays.

Authors: Berthier, Erwin; Guckenberger, David J; Cavnar, Peter; Huttenlocher, Anna; Keller, Nancy P; Beebe, David J

Published In Lab Chip, (2013 Feb 07)

Abstract: Microscale methods for cell-based assays typically rely on macroscopic reagent handling and fluidic loading protocols that are technically challenging and do not scale with the number of assays favorably. Here, we demonstrate a microfluidic platform technology called "Kit-On-A-Lid-Assay" (KOALA), that enables the creation of self-contained microfluidic cell-based assays, integrating all the steps required to perform cell-based assays. The KOALA platform allows the pre-packaging of reagents, cryopreservation of cell suspensions, thawing of cell suspensions, culture of cells, and operation of whole cell-based assays. The operation of the KOALA platform is user-friendly and consists of bringing together a lid containing the microchannels, and a base containing the pre-packaged reagents, thereby causing fluidic exchange in all the channels simultaneously. We demonstrate that the KOALA cell-based assays can be simply operated from start to finish without any external laboratory equipment.

PubMed ID: 23229806 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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