Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: HPLC-based assays for enzymes of glutathione biosynthesis.

Authors: White, C C; Krejsa, C J; Eaton, D L; Kavanagh, T J

Published In Curr Protoc Toxicol, (2001)

Abstract: Glutamate cysteine ligase and glutathione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.

PubMed ID: 23045057 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

Back
to Top