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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Identification of metabolic pathways influenced by the G-protein coupled receptors GprB and GprD in Aspergillus nidulans.

Authors: de Souza, Wagner R; Morais, Enyara Rezende; Krohn, Nadia Graciele; Savoldi, Marcela; Goldman, Maria Helena S; Rodrigues, Fernando; Caldana, Camila; Semelka, Charles T; Tikunov, Andrey P; Macdonald, Jeffrey M; Goldman, Gustavo Henrique

Published In PLoS One, (2013)

Abstract: Heterotrimeric G-protein-mediated signaling pathways play a pivotal role in transmembrane signaling in eukaryotes. Our main aim was to identify signaling pathways regulated by A. nidulans GprB and GprD G-protein coupled receptors (GPCRs). When these two null mutant strains were compared to the wild-type strain, the ýýgprB mutant showed an increased protein kinase A (PKA) activity while growing in glucose 1% and during starvation. In contrast, the ýýgprD has a much lower PKA activity upon starvation. Transcriptomics and (1)H NMR-based metabolomics were performed on two single null mutants grown on glucose. We noted modulation in the expression of 11 secondary metabolism gene clusters when the ýýgprB and ýýgprD mutant strains were grown in 1% glucose. Several members of the sterigmatocystin-aflatoxin gene cluster presented down-regulation in both mutant strains. The genes of the NR-PKS monodictyphenone biosynthesis cluster had overall increased mRNA accumulation in ýýgprB, while in the ýýgprD mutant strain the genes had decreased mRNA accumulation. Principal component analysis of the metabolomic data demonstrated that there was a significant metabolite shift in the ýýgprD strain. The (1)H NMR analysis revealed significant expression of essential amino acids with elevated levels in the ýýgprD strain, compared to the wild-type and ýýgprB strains. With the results, we demonstrated the differential expression of a variety of genes related mainly to secondary metabolism, sexual development, stress signaling, and amino acid metabolism. We propose that the absence of GPCRs triggered stress responses at the genetic level. The data suggested an intimate relationship among different G-protein coupled receptors, fine-tune regulation of secondary and amino acid metabolisms, and fungal development.

PubMed ID: 23658706 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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