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National Institute of Environmental Health Sciences

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Publication Detail

Title: Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures.

Authors: Oldenburg, Amy L; Chhetri, Raghav K; Cooper, Jason M; Wu, Wei-Chen; Troester, Melissa A; Tracy, Joseph B

Published In Opt Lett, (2013 Aug 01)

Abstract: We propose a method for differentiating classes of light scatterers based upon their temporal and polarization properties computed from time series of polarization-sensitive optical coherence tomography (PS-OCT) images. The amplitude (motility) and time scale (autocorrelation decay time) of the speckle fluctuations are combined with the cross-polarization pixel-wise to render Motility-, autocorrelation-, and polarization-sensitive (MAPS) OCT contrast images. This combination of metrics provides high specificity for discriminating diffusive gold nanorods and mammary epithelial cell spheroids within 3D tissue culture, based on their unique MAPS signature. This has implications toward highly specific contrast in molecular (nanoparticle-based) and functional (cellular activity) imaging using standard PS-OCT hardware.

PubMed ID: 23903180 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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