Title: Evaluation of neurobehavioral and neuroinflammatory end-points in the post-exposure period in rats sub-acutely exposed to manganese.
Authors: Santos, Dinamene; Dinamene, Santos; Batoréu, M Camila; Camila, Batoreu M; Tavares de Almeida, I; Davis Randall, L; Mateus, M Luísa; Luisa, Mateus M; Andrade, Vanda; Vanda, Andrade; Ramos, Ruben; Ruben, Ramos; Torres, Edite; Edite, Torres; Aschner, Michael; Michael, Aschner; Marreilha dos Santos, A P; Marreilha Dos Santos, A P
Published In Toxicology, (2013 Dec 06)
Abstract: Manganese (Mn) can cause manganism, a neurological disorder similar to Parkinson' Disease (PD). The neurobehavioral and neuroinflammatory end-points in the Mn post exposure period have not been studied yet. Rats were injected on alternate days with 8 doses of MnCl2 (25mg/kg) or saline, then euthanized 1, 10, 30 or 70 days following the last dose. Whole-blood (WB) (p<0.05), urine (p<0.05) and brain cortical (p<0.0001) Mn levels were significantly increased 24h after the last dose. Decreases in the rats' ambulation were noted 1, 10 and 30 days after the last Mn dose (p<0.001; p<0.05; p<0.001, respectively) and also in the rearing activity at the four time-points (p<0.05). Cortical glial fibrillary acid protein immunoreactivity (GFAP-ir) was significantly increased at 1, 10, 30 (p<0.0001) and 70 (p<0.001) days after the last Mn dose, as well as tumor necrosis α (TNF-α) levels (p<0.05) but just on day 1. Taken together, the results show that, during the 70-day clearance phase of Mn, the recovery is not immediate as behavioral alterations and neuroinflammation persist long after Mn is cleared from the cortical brain compartment.
PubMed ID: 24060432
MeSH Terms: Animals; Behavior, Animal/drug effects*; Brain/metabolism; Cerebral Cortex/chemistry; Cerebral Cortex/metabolism; Chlorides; Dose-Response Relationship, Drug; Endpoint Determination; Glial Fibrillary Acidic Protein/metabolism; Immunohistochemistry; Inflammation/chemically induced; Inflammation/pathology*; Male; Manganese Compounds; Manganese Poisoning/pathology*; Manganese Poisoning/psychology*; Manganese/blood; Manganese/metabolism; Manganese/urine; Motor Activity/drug effects; Rats; Rats, Wistar; Spectrophotometry, Atomic