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National Institute of Environmental Health Sciences

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Publication Detail

Title: Identification of alternatively spliced transcripts using a proteomic informatics approach.

Authors: Menon, Rajasree; Omenn, Gilbert S

Published In Methods Mol Biol, (2011)

Abstract: We present the protocol for the identification of alternatively spliced peptide sequences from tandem mass spectrometry datasets searched using X!Tandem against our modified ECgene resource with all potential translation products and then matched with the Michigan Peptide to Protein Integration (MPPI) scheme. This approach is suitable for human and mouse datasets. Application of the method is illustrated with a study of the Kras activation-Ink4/Arf deletion mouse model of human pancreatic ductal adenocarcinoma.

PubMed ID: 21063957 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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