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National Institute of Environmental Health Sciences

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Publication Detail

Title: Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase.

Authors: Chi, Peter; San Filippo, Joseph; Sehorn, Michael G; Petukhova, Galina V; Sung, Patrick

Published In Genes Dev, (2007 Jul 15)

Abstract: The HOP2 and MND1 genes are indispensable for meiotic recombination. The products of these genes associate to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of Rad51 and Dmc1. Here we conduct molecular studies to delineate the action mechanism of the Hop2-Mnd1 complex. We present evidence to implicate Hop2 as the major DNA-binding subunit and Mnd1 as the prominent Rad51 interaction entity. Hop2-Mnd1 stabilizes the Rad51-single-stranded DNA (ssDNA) nucleoprotein filament, the catalytic intermediate in recombination reactions. We also show that Hop2-Mnd1 enhances the ability of the Rad51-ssDNA nucleoprotein filament to capture duplex DNA, an obligatory step in the formation of the synaptic complex critical for DNA joint formation. Thus, our results unveil a bipartite mechanism of Hop2-Mnd1 in homologous DNA pairing: stabilization of the Rad51 presynaptic filament and duplex DNA capture to enhance synaptic complex formation.

PubMed ID: 17639080 Exiting the NIEHS site

MeSH Terms: Animals; Chromosomal Proteins, Non-Histone/chemistry; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism*; Chromosome Pairing; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/metabolism; Humans; Meiosis; Mice; Models, Biological; Multiprotein Complexes; Rad51 Recombinase/chemistry; Rad51 Recombinase/genetics; Rad51 Recombinase/metabolism*; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Recombination, Genetic; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism*

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