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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Improved protein identification through the use of unstained gels.

Authors: Loiselle, David R; Thelin, William R; Parker, Carol E; Dicheva, Nedyalka N; Kesner, Barry A; Mocanu, Viorel; Wang, Frank; Milgram, Sharon L; Warren, Maria R Esteban; Borchers, Christoph H

Published In J Proteome Res, (2005 May-Jun)

Abstract: In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silver-staining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data.

PubMed ID: 15952747 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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