Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Simple, High-Yield Syntheses of DNA Duplexes Containing Interstrand DNA-DNA Cross-Links Between an N(4) -Aminocytidine Residue and an Abasic Site.

Authors: Gamboa Varela, Jacqueline; Gates, Kent S

Published In Curr Protoc Nucleic Acid Chem, (2016 06 01)

Abstract: The protocol describes the preparation and purification of interstrand DNA-DNA cross-links derived from the reaction of an N(4) -aminocytidine residue with an abasic site in duplex DNA. The procedures employ inexpensive, commercially available chemicals and enzymes to carry out post-synthetic modification of commercially available oligodeoxynucleotides. The yield of cross-linked duplex is typically better than 90%. If purification is required, the cross-linked duplex can be readily separated from single-stranded DNA starting materials by denaturing gel electrophoresis. The resulting covalent hydrazone-based cross-links are stable under physiologically relevant conditions and may be useful for biophysical studies, structural analyses, DNA repair studies, and materials science applications. © 2016 by John Wiley & Sons, Inc.

PubMed ID: 27248783 Exiting the NIEHS site

MeSH Terms: Chemistry Techniques, Synthetic; Cross-Linking Reagents/chemistry; Cytidine/chemistry*; DNA/chemistry; Native Polyacrylamide Gel Electrophoresis; Nucleic Acid Heteroduplexes/chemistry*; Oligodeoxyribonucleotides/chemical synthesis; Oligodeoxyribonucleotides/chemistry*; Phosphorus Radioisotopes/chemistry

Back
to Top