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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Differential regulation of the anti-crossover and replication fork regression activities of Mph1 by Mte1.

Authors: Xue, Xiaoyu; Papusha, Alma; Choi, Koyi; Bonner, Jacob N; Kumar, Sandeep; Niu, Hengyao; Kaur, Hardeep; Zheng, Xiao-Feng; Donnianni, Roberto A; Lu, Lucy; Lichten, Michael; Zhao, Xiaolan; Ira, Grzegorz; Sung, Patrick

Published In Genes Dev, (2016 03 15)

Abstract: We identified Mte1 (Mph1-associated telomere maintenance protein 1) as a multifunctional regulator of Saccharomyces cerevisiae Mph1, a member of the FANCM family of DNA motor proteins important for DNA replication fork repair and crossover suppression during homologous recombination. We show that Mte1 interacts with Mph1 and DNA species that resemble a DNA replication fork and the D loop formed during recombination. Biochemically, Mte1 stimulates Mph1-mediated DNA replication fork regression and branch migration in a model substrate. Consistent with this activity, genetic analysis reveals that Mte1 functions with Mph1 and the associated MHF complex in replication fork repair. Surprisingly, Mte1 antagonizes the D-loop-dissociative activity of Mph1-MHF and exerts a procrossover role in mitotic recombination. We further show that the influence of Mte1 on Mph1 activities requires its binding to Mph1 and DNA. Thus, Mte1 differentially regulates Mph1 activities to achieve distinct outcomes in recombination and replication fork repair.

PubMed ID: 26966246 Exiting the NIEHS site

MeSH Terms: DEAD-box RNA Helicases/genetics; DEAD-box RNA Helicases/metabolism*; DNA Repair/genetics; DNA Replication/physiology*; Epistasis, Genetic; Gene Deletion; Gene Expression Regulation, Fungal; Mitosis; Protein Binding; Protein Multimerization; Protein Structure, Tertiary; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism*; Saccharomyces cerevisiae/genetics*; Saccharomyces cerevisiae/metabolism*; Telomere-Binding Proteins/metabolism*

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