Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Problems with the isolation of lymphoid clones with reactivity to human tumors.

Authors: Lotze, M T; Rayner, A A; Grimm, E A

Published In Behring Inst Mitt, (1985 Aug)

Abstract: The development of monospecific lymphoid reagents directed against autologous human tumors is a primary goal of our laboratory. Our approach using fresh human tumors as both targets in cytotoxicity assays and stimulators in proliferative assays with other appropriate controls makes possible a careful analysis of the reactivities present following either direct expansion in Interleukin 2 (IL-2) or following mixed lymphocyte tumor interaction and subsequent limiting dilution cloning. In addition, the activity of cells stimulated by IL-2 and subsequently cloned with fresh tumor lytic capability is discussed. These lymphokine activated killer (LAK) clones lyse multiple fresh human tumors but not fresh normal cells. The use of these expanded reagents with appropriate specificities may be useful in the adoptive immunotherapy of human malignancy. Our initial results as well as some of the difficulties associated with this approach are presented.

PubMed ID: 3936471 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

Back
to Top