Skip Navigation

Publication Detail

Title: The spleen as an extramedullary source of inflammatory cells responding to acetaminophen-induced liver injury.

Authors: Mandal, Mili; Gardner, Carol R; Sun, Richard; Choi, Hyejeong; Lad, Sonali; Mishin, Vladimir; Laskin, Jeffrey D; Laskin, Debra L

Published In Toxicol Appl Pharmacol, (2016 08 01)

Abstract: Macrophages have been shown to play a role in acetaminophen (APAP)-induced hepatotoxicity, contributing to both pro- and anti-inflammatory processes. In these studies, we analyzed the role of the spleen as an extramedullary source of hepatic macrophages. APAP administration (300mg/kg, i.p.) to control mice resulted in an increase in CD11b(+) infiltrating Ly6G(+) granulocytic and Ly6G(-) monocytic cells in the spleen and the liver. The majority of the Ly6G(+) cells were also positive for the monocyte/macrophage activation marker, Ly6C, suggesting a myeloid derived suppressor cell (MDSC) phenotype. By comparison, Ly6G(-) cells consisted of 3 subpopulations expressing high, intermediate, and low levels of Ly6C. Splenectomy was associated with increases in mature (F4/80(+)) and immature (F4/80(-)) pro-inflammatory Ly6C(hi) macrophages and mature anti-inflammatory (Ly6C(lo)) macrophages in the liver after APAP; increases in MDSCs were also noted in the livers of splenectomized (SPX) mice after APAP. This was associated with increases in APAP-induced expression of chemokine receptors regulating pro-inflammatory (CCR2) and anti-inflammatory (CX3CR1) macrophage trafficking. In contrast, APAP-induced increases in pro-inflammatory galectin-3(+) macrophages were blunted in livers of SPX mice relative to control mice, along with hepatic expression of TNF-α, as well as the anti-inflammatory macrophage markers, FIZZ-1 and YM-1. These data demonstrate that multiple subpopulations of pro- and anti-inflammatory cells respond to APAP-induced injury, and that these cells originate from distinct hematopoietic reservoirs.

PubMed ID: 27163765 Exiting the NIEHS site

MeSH Terms: Acetaminophen/toxicity*; Animals; CX3C Chemokine Receptor 1; Chemical and Drug Induced Liver Injury/physiopathology*; Chemokines/biosynthesis; Galectin 3/metabolism; Inflammation Mediators/metabolism*; Macrophage Activation/drug effects; Male; Mice; Mice, Inbred C57BL; Microsomes, Liver/drug effects; Myeloid Cells/drug effects*; Phenotype; Receptors, CCR2/biosynthesis; Receptors, Chemokine/biosynthesis; Spleen/metabolism*; Splenectomy

Back
to Top