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National Institute of Environmental Health Sciences

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Publication Detail

Title: Immunocytochemical localization of C-myc and C-jun oncoproteins in hamster kidney and estrogen-induced kidney tumors.

Authors: Bhat, H K; Springer, I; Rajaraman, S; Liehr, J G

Published In J Steroid Biochem Mol Biol, (1997 Jan)

Abstract: The chronic administration of 17beta-estradiol to male Syrian hamsters for 6-7 months induces kidney tumors which express high levels of c-fos, c-myc and c-jun mRNA compared to surrounding tissue or untreated controls. In this study, we have investigated, by immunocytochemical methods, the cellular localization of c-myc and c-jun oncoproteins in estrogen-dependent kidney tumors, in kidney tissue of hamsters treated with 17beta-estradiol for 6 months and in the kidneys of age-matched controls. The c-myc oncoprotein was strongly expressed in tumors, in smooth muscle layers of arteries and in parietal epithelial cells of the glomerulus. In age-matched untreated kidneys, there was little or no staining in the glomerulus, arteries or kidney tubular cells. The c-jun oncoprotein was detected in kidney tumors and in the tubular epithelium of surrounding tissue. The immunoreactivity for c-jun oncoprotein was highest in the tumor, intermediate in estrogen-treated kidney tissue and lowest in kidney tubular cells of controls. It is concluded that the high expression of c-myc in estrogen-induced kidney tumors, in the smooth muscle layer of arteries, and in glomerular parietal epithelial cells in the kidneys of 17beta-estradiol-treated hamsters, but poor expression in control kidneys indicate an involvement of this oncoprotein in the tumorigenic process. In contrast, c-jun is expressed in untreated, in 17beta-estradiol-treated kidneys and in tumors, and may not serve as a prognostic marker in the transformation of these cells to the malignant phenotype.

PubMed ID: 9182863 Exiting the NIEHS site

MeSH Terms: Animals; Cricetinae; Estradiol/pharmacology; Estrogens/pharmacology*; Immunohistochemistry; Kidney Neoplasms/chemically induced; Kidney Neoplasms/genetics*; Kidney/physiology*; Male; Proto-Oncogene Proteins c-jun/analysis*; Proto-Oncogene Proteins c-jun/drug effects; Proto-Oncogene Proteins c-jun/immunology; Proto-Oncogene Proteins c-myc/analysis*; Proto-Oncogene Proteins c-myc/drug effects; Proto-Oncogene Proteins c-myc/immunology

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