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Publication Detail

Title: Human RAD52 interactions with replication protein A and the RAD51 presynaptic complex.

Authors: Ma, Chu Jian; Kwon, Youngho; Sung, Patrick; Greene, Eric C

Published In J Biol Chem, (2017 07 14)

Abstract: Rad52 is a highly conserved protein involved in the repair of DNA damage. Human RAD52 has been shown to mediate single-stranded DNA (ssDNA) and is synthetic lethal with mutations in other key recombination proteins. For this study, we used single-molecule imaging and ssDNA curtains to examine the binding interactions of human RAD52 with replication protein A (RPA)-coated ssDNA, and we monitored the fate of RAD52 during assembly of the presynaptic complex. We show that RAD52 binds tightly to the RPA-ssDNA complex and imparts an inhibitory effect on RPA turnover. We also found that during presynaptic complex assembly, most of the RPA and RAD52 was displaced from the ssDNA, but some RAD52-RPA-ssDNA complexes persisted as interspersed clusters surrounded by RAD51 filaments. Once assembled, the presence of RAD51 restricted formation of new RAD52-binding events, but additional RAD52 could bind once RAD51 dissociated from the ssDNA. Together, these results provide new insights into the behavior and dynamics of human RAD52 during presynaptic complex assembly and disassembly.

PubMed ID: 28551686 Exiting the NIEHS site

MeSH Terms: DNA, Single-Stranded/metabolism*; Enzyme Stability; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Humans; Kinetics; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Microscopy, Fluorescence; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Presynaptic Terminals/enzymology; Presynaptic Terminals/metabolism*; Protein Multimerization; Protein Stability; Rad51 Recombinase/chemistry; Rad51 Recombinase/genetics; Rad51 Recombinase/metabolism*; Rad52 DNA Repair and Recombination Protein/chemistry; Rad52 DNA Repair and Recombination Protein/genetics; Rad52 DNA Repair and Recombination Protein/metabolism*; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Replication Protein A/chemistry; Replication Protein A/genetics; Replication Protein A/metabolism*

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