Title: Quantification of manganese and mercury in toenail in vivo using portable X-ray fluorescence (XRF).

Authors: Zhang, Xinxin; Specht, Aaron J; Weisskopf, Marc G; Weuve, Jennifer; Nie, Linda H

Published In Biomarkers, (2018 Mar)

Abstract: Toenail is an advantageous biomarker to assess exposure to metals such as manganese and mercury. Toenail Mn and Hg are in general analyzed by chemical methods such as inductively coupled plasma mass spectrometry and atomic absorption spectrophotometry. In this project, a practical and convenient technology-portable X-ray florescence (XRF)-is studied for the noninvasive in vivo quantification of manganese and mercury in toenail.The portable XRF method has advantages in that it does not require toenail clipping and it can be done in 3 min, which will greatly benefit human studies involving the assessment of manganese and mercury exposures. This study mainly focused on the methodology development and validation which includes spectral analysis, system calibration, the effect of toenail thickness, and the detection limit of the system. Manganese- and mercury-doped toenail phantoms were made. Calibration lines were established for these measurements.The results show that the detection limit for manganese is 3.65 μg/g (ppm) and for mercury is 0.55 μg/g (ppm) using 1 mm thick nail phantoms with 10 mm soft tissue underneath.We conclude that portable XRF is a valuable and sensitive technology to quantify toenail manganese and mercury in vivo.

PubMed ID: 28901783

MeSH Terms: No MeSH terms associated with this publication