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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Integrating DNA Methylation and Hydroxymethylation Data with the Mint Pipeline.

Authors: Cavalcante, Raymond G; Patil, Snehal; Park, Yongseok; Rozek, Laura S; Sartor, Maureen A

Published In Cancer Res, (2017 11 01)

Abstract: DNA methylation (5mC) plays important roles in mammalian development, oncogenesis, treatment response, and responses to the environment. DNA hydroxymethylation (5hmC) is also an informative epigenetic mark with distinct roles in regulation and cancer. Gold-standard, widely used technologies (bisulfite conversion, followed by deep sequencing) cannot distinguish between 5mC and 5hmC. Therefore, additional experiments are required to differentiate the two marks, and in silico methods are needed to analyze, integrate, and interpret these data. We developed the Methylation INTegration (mint) pipeline to support the comprehensive analysis of bisulfite conversion and immunoprecipitation-based methylation and hydroxymethylation assays, with additional steps toward integration, visualization, and interpretation. The pipeline is available as both a command line and a Galaxy graphical user interface tool. Both implementations require minimal configuration while remaining flexible to experiment specific needs. Cancer Res; 77(21); e27-30. ©2017 AACR.

PubMed ID: 29092933 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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