Title: miR-500a-5p regulates oxidative stress response genes in breast cancer and predicts cancer survival.

Authors: Degli Esposti, Davide; Aushev, Vasily N; Lee, Eunjee; Cros, Marie-Pierre; Zhu, Jun; Herceg, Zdenko; Chen, Jia; Hernandez-Vargas, Hector

Published In Sci Rep, (2017 Nov 21)

Abstract: MicroRNAs (miRNAs) are small regulatory non-coding RNAs with a diversity of cellular functions, and are frequently dysregulated in cancer. Using a novel computational method (ActMir) that we recently developed, the "activity" of miRNA hsa-miR-500a was implicated in estrogen receptor (ER) positive breast cancer; however its targets and functional impact remain poorly understood. Here, we performed an extensive gene expression analysis in ER+ breast cancer cell lines, to reveal the targets of miR-500a-5p after experimental modulation of its levels. We found that among mRNAs targeted by miR-500a-5p there was enrichment in oxidative stress response genes. Moreover, in vitro exposure to oxidative stress using H2O2 induces miR-500a-5p overexpression and downregulation of the oxidative stress targets TXNRD1 and NFE2L2. Finally, expression of several of the identified miR-500a-5p targets related to oxidative stress, including TXNRD1, was associated with ER+ breast cancer survival in multiple datasets. Overall, we identify miR-500a-5p as an oxidative stress response miRNA whose activity may define breast cancer progression and survival.

PubMed ID: 29162888

MeSH Terms: No MeSH terms associated with this publication