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Publication Detail

Title: miFAST: A novel and rapid microRNA target capture method.

Authors: Poenitzsch Strong, Ashley M; Berry, Scott M; Beebe, David J; Li, Jian-Liang; Spiegelman, Vladimir S

Published In Mol Carcinog, (2018 04)

Abstract: MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.

PubMed ID: 29350431 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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