Title: In trans hydrolysis of carrier protein-bound acyl intermediates by CitA during citrinin biosynthesis.
Authors: Storm, Philip A; Townsend, Craig A
Published In Chem Commun (Camb), (2017 Dec 19)
Abstract: Polyketide synthases (PKSs) have several known editing mechanisms to ensure that non-productive intermediates are removed from the acyl carrier protein (ACP). We demonstrate that CitA, a putative hydrolase in the citrinin biosynthetic gene cluster, removes ACP-bound acyl intermediates. We propose that it serves an editing role in trans.
PubMed ID: 29189834
MeSH Terms: Acyl Carrier Protein/metabolism; Citrinin/biosynthesis*; Citrinin/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism*; Hydrolases/genetics; Hydrolases/metabolism*; Hydrolysis; Isomerism; Monascus/enzymology; Mutagenesis, Site-Directed; Polyketide Synthases/metabolism