Title: Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo.
Authors: Jung, Yoon Hee; Kremsky, Isaac; Gold, Hannah B; Rowley, M Jordan; Punyawai, Kanchana; Buonanotte, Alyx; Lyu, Xiaowen; Bixler, Brianna J; Chan, Anthony W S; Corces, Victor G
Published In Mol Cell, (2019 07 11)
Abstract: The epigenetic information present in mammalian gametes and whether it is transmitted to the progeny are relatively unknown. We find that many promoters in mouse sperm are occupied by RNA polymerase II (Pol II) and Mediator. The same promoters are accessible in GV and MII oocytes and preimplantation embryos. Sperm distal ATAC-seq sites containing motifs for various transcription factors are conserved in monkeys and humans. ChIP-seq analyses confirm that Foxa1, ERα, and AR occupy distal enhancers in sperm. Accessible sperm enhancers containing H3.3 and H2A.Z are also accessible in oocytes and preimplantation embryos. Furthermore, their interactions with promoters in the gametes persist during early development. Sperm- or oocyte-specific interactions mediated by CTCF and cohesin are only present in the paternal or maternal chromosomes, respectively, in the zygote and 2-cell stages. These interactions converge in both chromosomes by the 8-cell stage. Thus, mammalian gametes contain complex patterns of 3D interactions that can be transmitted to the zygote after fertilization.
PubMed ID: 31056445
MeSH Terms: Animals; Base Sequence; CCCTC-Binding Factor/genetics*; CCCTC-Binding Factor/metabolism; Chromatin/chemistry; Chromatin/metabolism; Conserved Sequence; Embryo, Mammalian; Embryonic Development/genetics; Enhancer Elements, Genetic; Estrogen Receptor alpha/genetics; Estrogen Receptor alpha/metabolism; Estrogen Receptor beta/genetics; Estrogen Receptor beta/metabolism; Female; Gene Expression Regulation, Developmental; Hepatocyte Nuclear Factor 3-beta/genetics*; Hepatocyte Nuclear Factor 3-beta/metabolism; Humans; Macaca mulatta; Male; Mice; Oocytes/cytology; Oocytes/growth & development; Oocytes/metabolism*; Receptors, Androgen/genetics; Receptors, Androgen/metabolism; Sequence Homology, Nucleic Acid; Spermatozoa/cytology; Spermatozoa/growth & development; Spermatozoa/metabolism*; Zinc Fingers/genetics; Zygote/cytology; Zygote/growth & development; Zygote/metabolism*