Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Nicking enzyme-assisted signal-amplifiable Hg2+ detection using upconversion nanoparticles.

Authors: Vijayan, Anjaly N; Liu, Zhiming; Zhao, Haohan; Zhang, Peng

Published In Anal Chim Acta, (2019 Sep 23)

Abstract: A highly specific and sensitive isothermal method for mercury detection using DNA-conjugated upconversion nanoparticles is reported. A single-stranded DNA containing thymine bases, used as the Hg2+-capturing element through the formation of thymine-Hg2+-thymine complex, is covalently attached to the NaYF4: Yb3+, Tm3+ nanoparticles. Luminescence resonance energy transfer takes place between the NaYF4: Yb3+, Tm3+ nanoparticles as donor and DNA-intercalating SYBR Green I as the acceptor upon excitation of 980 nm. The sensitivity and selectivity toward Hg2+ are enhanced using the nicking enzyme, Nt. Alwl, which leads to signal amplification. By monitoring the ratio of acceptor emission to a reference peak, the presence of Hg2+ ions are quantitatively determined with a lower detection limit of 0.14 nM, which is much lower than the US Environmental Protection Agency (EPA) limit of Hg2+ in drinking water.

PubMed ID: 31146867 Exiting the NIEHS site

MeSH Terms: DNA/chemistry*; Drinking Water/chemistry; Fluorescence Resonance Energy Transfer; Mercury/analysis*; Nanoparticles/chemistry*; Nucleic Acid Amplification Techniques*

Back
to Top
Last Reviewed: October 07, 2024