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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Feasibility of the Bag-Mediated Filtration System for Environmental Surveillance of Poliovirus in Kenya.

Authors: Zhou, Nicolette A; Fagnant-Sperati, Christine S; Komen, Evans; Mwangi, Benlick; Mukubi, Johnstone; Nyangao, James; Hassan, Joanne; Chepkurui, Agnes; Maina, Caroline; van Zyl, Walda B; Matsapola, Peter N; Wolfaardt, Marianne; Ngwana, Fhatuwani B; Jeffries-Miles, Stacey; Coulliette-Salmond, Angela; Peñaranda, Silvia; Shirai, Jeffry H; Kossik, Alexandra L; Beck, Nicola K; Wilmouth, Robyn; Boyle, David S; Burns, Cara C; Taylor, Maureen B; Borus, Peter; Meschke, John Scott

Published In Food Environ Virol, (2020 03)

Abstract: The bag-mediated filtration system (BMFS) was developed to facilitate poliovirus (PV) environmental surveillance, a supplement to acute flaccid paralysis surveillance in PV eradication efforts. From April to September 2015, environmental samples were collected from four sites in Nairobi, Kenya, and processed using two collection/concentration methodologies: BMFS (> 3 L filtered) and grab sample (1 L collected; 0.5 L concentrated) with two-phase separation. BMFS and two-phase samples were analyzed for PV by the standard World Health Organization poliovirus isolation algorithm followed by intratypic differentiation. BMFS samples were also analyzed by a cell culture independent real-time reverse transcription polymerase chain reaction (rRT-PCR) and an alternative cell culture method (integrated cell culture-rRT-PCR with PLC/PRF/5, L20B, and BGM cell lines). Sabin polioviruses were detected in a majority of samples using BMFS (37/42) and two-phase separation (32/42). There was statistically more frequent detection of Sabin-like PV type 3 in samples concentrated with BMFS (22/42) than by two-phase separation (14/42, p = 0.035), possibly due to greater effective volume assayed (870 mL vs. 150 mL). Despite this effective volume assayed, there was no statistical difference in Sabin-like PV type 1 and Sabin-like PV type 2 detection between these methods (9/42 vs. 8/42, p = 0.80 and 27/42 vs. 32/42, p = 0.18, respectively). This study demonstrated that BMFS can be used for PV environmental surveillance and established a feasible study design for future research.

PubMed ID: 31679104 Exiting the NIEHS site

MeSH Terms: Environmental Monitoring/instrumentation; Environmental Monitoring/methods*; Feasibility Studies; Filtration/instrumentation; Filtration/methods*; Fresh Water/chemistry; Fresh Water/virology*; Humans; Kenya; Poliomyelitis/virology; Poliovirus/classification; Poliovirus/genetics; Poliovirus/isolation & purification*

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