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Title: Nickel-Induced Developmental Neurotoxicity in C. elegans Includes Cholinergic, Dopaminergic and GABAergic Degeneration, Altered Behaviour, and Increased SKN-1 Activity.

Authors: Ijomone, Omamuyovwi M; Miah, Mahfuzur R; Akingbade, Grace T; Bucinca, Hana; Aschner, Michael

Published In Neurotox Res, (2020 Apr)

Abstract: Nickel (Ni) is a ubiquitous metal in the environment with increasing industrial application. While environmental and occupational exposure to Ni compounds has been known to result in toxicities to several organs, including the liver, kidney, lungs, skin and gonads, neurotoxic effects have not been extensively investigated. In this present study, we investigated specific neuronal susceptibility in a C. elegans model of acute Ni neurotoxicity. Wild-type worms and worms expressing green fluorescent protein (GFP) in either cholinergic, dopaminergic or GABAergic neurons were treated with NiCl2 for 1 h at the first larval (L1) stage. The median lethal dose (LD50) was calculated to be 5.88 mM in this paradigm. Morphology studies of GFP-expressing worms showed significantly increasing degeneration of cholinergic, dopaminergic and GABAergic neurons with increasing Ni concentration. Significant functional changes in locomotion and basal slowing response assays reflected that cholinergic and dopaminergic neuronal function, respectively, were impaired due to Ni treatment. Interestingly, a small but significant number of worms exhibited shrinker phenotype upon Ni exposure but no loopy head foraging behaviour was observed suggesting that function of D-type GABAergic neurons of C elegans may be specifically attenuated while the RME subset of GABAergic neurons are not. GFP expression due to induction of glutathione S-transferase 4 (gst-4), a target of Nrf2 homolog skn-1, was increased in a Pgst-4::GFP worm highlighting Ni-induced oxidative stress. RT-qPCR verified upregulation of this expression of gst-4 immediately after exposure. These data suggest that oxidative stress is associated with neuronal damage and altered behaviour due to developmental Ni exposure.

PubMed ID: 32034695 Exiting the NIEHS site

MeSH Terms: Animals; Animals, Genetically Modified; Caenorhabditis elegans; Caenorhabditis elegans Proteins/biosynthesis*; Caenorhabditis elegans Proteins/genetics; Cholinergic Neurons/drug effects; Cholinergic Neurons/metabolism*; DNA-Binding Proteins/biosynthesis*; DNA-Binding Proteins/genetics; Dopaminergic Neurons/drug effects; Dopaminergic Neurons/metabolism*; Dose-Response Relationship, Drug; GABAergic Neurons/drug effects; GABAergic Neurons/metabolism*; Green Fluorescent Proteins/biosynthesis; Green Fluorescent Proteins/genetics; Locomotion/drug effects; Locomotion/physiology; Nerve Degeneration/chemically induced; Nerve Degeneration/genetics; Nerve Degeneration/metabolism*; Nickel/toxicity*; Transcription Factors/biosynthesis*; Transcription Factors/genetics

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Last Reviewed: October 02, 2024