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Publication Detail

Title: Activation of β-Catenin Signaling and its Crosstalk With Estrogen and Histone Deacetylases in Human Uterine Fibroids.

Authors: Ali, Mohamed; Shahin, Sara Mahmoud; Sabri, Nagwa Ali; Al-Hendy, Ayman; Yang, Qiwei

Published In J Clin Endocrinol Metab, (2020 04 01)

Abstract: Uterine fibroids (UF) are the most common benign tumor of the myometrium (MM) in women of reproductive age. However, the mechanism underlying the pathogenesis of UF is largely unknown.To explore the link between nuclear β-catenin and UF phenotype and β-catenin crosstalk with estrogen and histone deacetylases (HDACs).Protein/RNA levels of β-catenin (CTNNB1 gene), its responsive markers cyclin D1 and c-Myc, androgen receptor (AR), p27, and class-I HDACs were measured in matched UF/MM tissues or cell populations. The effects of chemical inhibition/activation and genetic knockdown of CTNNB1 on UF phenotype were measured. The anti-UF effect of 2 HDAC inhibitors was evaluated.β-catenin nuclear translocation in response to β-catenin inhibition/activation, estrogen, and HDAC inhibitors in UF cells.UF tissues/cells showed significantly higher expression of nuclear β-catenin, cyclin D1, c-Myc, and HDACs 1, 2, 3, and 8 than MM. Estradiol induced β-catenin nuclear translocation and consequently its responsive genes in both MM and UF cells, while an estrogen receptor antagonist reversed this induction effect. Treatment with β-catenin or HDAC inhibitors led to dose-dependent growth inhibition, while Wnt3a treatment increased proliferation compared with control. Chemical inhibition of β-catenin decreased cyclin D1 and c-Myc expression levels, while β-catenin activation increased expression of the same markers. Genetic knockdown of CTNNB1 resulted in a marked decrease in β-catenin, cyclin D1, c-Myc, and AR expression. Treatment of UF cells with HDAC inhibitors decreased nuclear β-catenin, cyclin D1, and c-Myc expression. Moreover, HDAC inhibitors induced apoptosis of UF cells and cell cycle arrest.β-catenin nuclear translocation contributes to UF phenotype, and β-catenin signaling is modulated by estradiol and HDAC activity.

PubMed ID: 31761932 Exiting the NIEHS site

MeSH Terms: Biomarkers/metabolism*; Cyclin D1/genetics; Cyclin D1/metabolism; Estrogens/pharmacology*; Female; Histone Deacetylase 1/antagonists & inhibitors; Histone Deacetylase 1/genetics; Histone Deacetylase 1/metabolism*; Histone Deacetylase Inhibitors/pharmacology; Humans; Leiomyoma/drug therapy; Leiomyoma/metabolism; Leiomyoma/pathology*; Receptors, Androgen/genetics; Receptors, Androgen/metabolism; Receptors, Estrogen/genetics; Receptors, Estrogen/metabolism*; Signal Transduction; Tumor Cells, Cultured; beta Catenin/genetics; beta Catenin/metabolism*

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