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Title: Analysis of Telomere Length and Aberrations by Quantitative FISH.

Authors: Fouquerel, Elise; Opresko, Patricia

Published In Methods Mol Biol, (2020)

Abstract: A key component of sustained cellular proliferation is the preservation of telomere integrity. Telomeres are nucleoprotein structures that cap and protect linear chromosomes. Their linearity and repetitive sequence represent a challenge for the replication machinery and cause telomere shortening, fragility, and losses. Here we describe the common technique of quantitative fluorescent in situ hybridization that allows for the scoring of telomere aberrations and measurement of telomere length directly on metaphase chromosomes through the use of highly specific peptide nucleic acid probes.

PubMed ID: 31989559 Exiting the NIEHS site

MeSH Terms: Animals; Cells, Cultured; Humans; In Situ Hybridization, Fluorescence/methods*; Metaphase; Microscopy, Fluorescence; Nucleic Acid Probes; Peptide Nucleic Acids/chemistry; Peptide Nucleic Acids/genetics; Telomere Homeostasis; Telomere/genetics*; Workflow

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