Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples.

Authors: Chen, Zi-Jian; Liu, Xi-Xia; Xiao, Zhi-Li; Fu, Hui-Jun; Huang, Yu-Ping; Huang, Shu-Yi; Shen, Yu-Dong; He, Fan; Yang, Xing-Xing; Hammock, Bruce; Xu, Zhen-Lin

Published In Ecotoxicol Environ Saf, (2020 Jun 15)

Abstract: 1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.

PubMed ID: 32247241 Exiting the NIEHS site

MeSH Terms: Antibodies, Monoclonal/chemistry*; Antibodies, Monoclonal/immunology; Carbaryl/metabolism; Environmental Exposure/analysis; Enzyme-Linked Immunosorbent Assay/methods*; Haptens/chemistry*; Limit of Detection; Naphthalenes/metabolism; Naphthols/immunology; Naphthols/urine*; Pesticide Residues/immunology; Pesticide Residues/metabolism; Pesticide Residues/urine*

to Top