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Publication Detail

Title: Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples.

Authors: Chen, Zi-Jian; Liu, Xi-Xia; Xiao, Zhi-Li; Fu, Hui-Jun; Huang, Yu-Ping; Huang, Shu-Yi; Shen, Yu-Dong; He, Fan; Yang, Xing-Xing; Hammock, Bruce; Xu, Zhen-Lin

Published In Ecotoxicol Environ Saf, (2020 Jun 15)

Abstract: 1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.

PubMed ID: 32247241 Exiting the NIEHS site

MeSH Terms: Antibodies, Monoclonal/chemistry*; Antibodies, Monoclonal/immunology; Carbaryl/metabolism; Environmental Exposure/analysis; Enzyme-Linked Immunosorbent Assay/methods*; Haptens/chemistry*; Limit of Detection; Naphthalenes/metabolism; Naphthols/immunology; Naphthols/urine*; Pesticide Residues/immunology; Pesticide Residues/metabolism; Pesticide Residues/urine*

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