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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Single-Cell Transcriptional Profiling of Cells Derived From Regenerating Alveolar Ducts.

Authors: Ysasi, Alexandra B; Bennett, Robert D; Wagner, Willi; Valenzuela, Cristian D; Servais, Andrew B; Tsuda, Akira; Pyne, Saumyadipta; Li, Shuqiang; Grimsby, Jonna; Pokharel, Prapti; Livak, Kenneth J; Ackermann, Maximilian; Blainey, Paul C; Mentzer, Steven J

Published In Front Med (Lausanne), (2020)

Abstract: Lung regeneration occurs in a variety of adult mammals after surgical removal of one lung (pneumonectomy). Previous studies of murine post-pneumonectomy lung growth have identified regenerative "hotspots" in subpleural alveolar ducts; however, the cell-types participating in this process remain unclear. To identify the single cells participating in post-pneumonectomy lung growth, we used laser microdissection, enzymatic digestion and microfluidic isolation. Single-cell transcriptional analysis of the murine alveolar duct cells was performed using the C1 integrated fluidic circuit (Fluidigm) and a custom PCR panel designed for lung growth and repair genes. The multi-dimensional data set was analyzed using visualization software based on the tSNE algorithm. The analysis identified 6 cell clusters; 1 cell cluster was present only after pneumonectomy. This post-pneumonectomy cluster was significantly less transcriptionally active than 3 other clusters and may represent a transitional cell population. A provisional cluster identity for 4 of the 6 cell clusters was obtained by embedding bulk transcriptional data into the tSNE analysis. The transcriptional pattern of the 6 clusters was further analyzed for genes associated with lung repair, matrix production, and angiogenesis. The data demonstrated that multiple cell-types (clusters) transcribed genes linked to these basic functions. We conclude that the coordinated gene expression across multiple cell clusters is likely a response to a shared regenerative microenvironment within the subpleural alveolar ducts.

PubMed ID: 32373614 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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