Title: Validation of the bag-mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya.

Authors: Fagnant-Sperati, C S; Ren, Y; Zhou, N A; Komen, E; Mwangi, B; Hassan, J; Chepkurui, A; Nzunza, R; Nyangao, J; van Zyl, W B; Wolfaardt, M; Matsapola, P N; Ngwana, F B; Jeffries-Miles, S; Coulliette-Salmond, A; Peñaranda, S; Vega, E; Shirai, J H; Kossik, A L; Beck, N K; Boyle, D S; Burns, C C; Taylor, M B; Borus, P; Meschke, J S

Published In J Appl Microbiol, (2021 Mar)

Abstract: AIMS: This study compared the bag-mediated filtration system (BMFS) and standard WHO two-phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin-like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016. METHODS AND RESULTS: Environmental samples were collected in Nairobi, Kenya (Sept 2015-Feb 2017), concentrated via BMFS and two-phase separation methods, then assayed using the WHO PV isolation algorithm and intratypic differentiation diagnostic screening kit. SL1, SL2 and SL3 were detected at higher rates in BMFS than two-phase samples (P < 0·05). In BMFS samples, SL PV detection did not significantly differ with volume filtered, filtration time or filter shipment time (P > 0·05), while SL3 was detected less frequently with higher shipment temperatures (P = 0·027). SL2 was detected more frequently before OPV2 withdrawal in BMFS and two-phase samples (P < 1 × 10-5 ). CONCLUSIONS: Poliovirus was detected at higher rates with the BMFS, a method that includes a secondary concentration step, than using the standard WHO two-phase method. SL2 disappearance from the environment was commensurate with OPV2 withdrawal. SIGNIFICANCE AND IMPACT OF THE STUDY: The BMFS offers comparable or improved PV detection under the conditions in this study, relative to the two-phase method.

PubMed ID: 32743931

MeSH Terms: No MeSH terms associated with this publication