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Title: Induction of prolactin expression and release in human preadipocytes by cAMP activating ligands.

Authors: McFarland-Mancini, Molly; Hugo, Eric; Loftus, Jean; Ben-Jonathan, Nira

Published In Biochem Biophys Res Commun, (2006 May 26)

Abstract: In addition to the pituitary, prolactin (PRL) in humans is produced at non-pituitary sites where it acts as a cytokine. We previously reported that PRL is expressed and released from breast adipose explants, raising the question as to the dynamics of its production and its regulation. Preadipocytes were isolated from breast adipose tissue obtained during breast reduction. PRL expression was transiently increased during early preadipocyte differentiation. Both isoproterenol, a beta-adrenergic receptor agonist, and PACAP, pituitary adenylate cyclase activating peptide, increased PRL expression, and release from preadipocytes. This stimulation was suppressed by several protein kinase inhibitors, suggesting involvement of multiple signaling pathways. Transfection of preadipocytes with a superdistal PRL promoter/luciferase reporter revealed two stimulatory domains and an inhibitory domain. These data establish the transcriptional regulation of adipocyte PRL by the superdistal PRL promoter, its transient expression during adipogenesis, and the stimulatory effect of catecholamines and PACAP.

PubMed ID: 16630538 Exiting the NIEHS site

MeSH Terms: Adipocytes/cytology*; Adipocytes/drug effects; Adipocytes/metabolism; Adipose Tissue/cytology; Adipose Tissue/metabolism; Adrenergic beta-Agonists/pharmacology; Biological Assay; Breast/cytology; Cell Differentiation/genetics*; Cells, Cultured; Cyclic AMP/pharmacology; Female; Gene Expression Regulation, Developmental*; Humans; Isoproterenol/pharmacology; Ligands; Pituitary Adenylate Cyclase-Activating Polypeptide/pharmacology; Prolactin/genetics*; Prolactin/metabolism*; Promoter Regions (Genetics)/drug effects; Protein Kinase Inhibitors/pharmacology; Protein Kinases/drug effects; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Trans-Activation (Genetics)*; Transcription, Genetic/drug effects

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