Title: Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy.

Authors: Xu, Bojie; Wang, Kai; Vasylieva, Natalia; Zhou, Hang; Xue, Xianle; Wang, Baomin; Li, Qing X; Hammock, Bruce D; Xu, Ting

Published In Anal Bioanal Chem, (2021 Apr)

Abstract: Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELISAs) for the detection of the two insecticides were developed. The half-maximum signal inhibition concentrations (IC50) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL-1, respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (Brassica chinensis L.) and soil samples were 90-129%, while those of chlorantraniliprole were in a range of 89-120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment.

PubMed ID: 33580830

MeSH Terms: Brassica/chemistry; Enzyme-Linked Immunosorbent Assay/methods*; Insecticides/analysis*; Pyrazoles/analysis*; Soil Pollutants/analysis*; Soil/chemistry; Vegetables/chemistry; ortho-Aminobenzoates/analysis*