Title: DNA Polymerase II Supports the Replicative Bypass of N2-Alkyl-2'-deoxyguanosine Lesions in Escherichia coli Cells.

Authors: Wang, Yinan; Wu, Jun; Wu, Jiabin; Wang, Yinsheng

Published In Chem Res Toxicol, (2021 Mar 15)

Abstract: Alkylation represents a main form of DNA damage. The N2 position of guanine is frequently alkylated in DNA. The SOS-induced polymerases have been shown to be capable of bypassing various DNA damage products in Escherichia coli. Herein, we explored the influences of four N2-alkyl-dG lesions (alkyl = ethyl, n-butyl, isobutyl, or sec-butyl) on DNA replication in AB1157 E. coli cells and the corresponding strains with polymerases (Pol) II, IV, and V being individually or simultaneously knocked out. We found that N2-Et-dG is slightly less blocking to DNA replication than the N2-Bu-dG lesions, which display very similar replication bypass efficiencies. Additionally, Pol II and, to a lesser degree, Pol IV and Pol V are required for the efficient bypass of the N2-alkyl-dG adducts, and none of these lesions was mutagenic. Together, our results support that the efficient replication across small N2-alkyl-dG DNA adducts in E. coli depends mainly on Pol II.

PubMed ID: 33417436

MeSH Terms: DNA Adducts/chemistry; DNA Adducts/metabolism*; DNA Polymerase II/metabolism*; DNA Replication; DNA, Bacterial/metabolism*; Deoxyguanosine/analogs & derivatives; Deoxyguanosine/chemistry; Deoxyguanosine/metabolism*; Escherichia coli/cytology; Escherichia coli/metabolism*; Molecular Structure