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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid.

Authors: Zhang, Can; Wu, Xiaoxiao; Li, Dongyang; Hu, Jinnuo; Wan, Debin; Zhang, Zhen; Hammock, Bruce D

Published In Anal Methods, (2021 04 14)

Abstract: As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL-1 and 0.1 ng mL-1, respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.

PubMed ID: 33861243 Exiting the NIEHS site

MeSH Terms: Benzoates; Enzyme-Linked Immunosorbent Assay; Gold*; Humans; Immunoassay; Metal Nanoparticles*

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