Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: RecA acts in trans to allow replication of damaged DNA by DNA polymerase V.

Authors: Schlacher, Katharina; Cox, Michael M; Woodgate, Roger; Goodman, Myron F

Published In Nature, (2006 Aug 24)

Abstract: The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage. Previously, it has been assumed that RecA binds to the DNA template strand being copied. Here we show, however, that pol-V-catalysed translesion synthesis, in the presence or absence of the beta-processivity-clamp, occurs only when RecA nucleoprotein filaments assemble or RecA protomers bind on separate single-stranded (ss)DNA molecules in trans. A 3'-proximal RecA filament end on trans DNA is essential for stimulation; however, synthesis is strengthened by further pol V-RecA interactions occurring elsewhere along a trans nucleoprotein filament. We suggest that trans-stimulation of pol V by RecA bound to ssDNA reflects a distinctive regulatory mechanism of mutation that resolves the paradox of RecA filaments assembled in cis on a damaged template strand obstructing translesion DNA synthesis despite the absolute requirement of RecA for SOS mutagenesis.

PubMed ID: 16929290 Exiting the NIEHS site

MeSH Terms: Catalysis; DNA Damage*; DNA Replication*; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; DNA-Directed DNA Polymerase/metabolism*; Escherichia coli Proteins; Escherichia coli/enzymology; Escherichia coli/genetics*; Escherichia coli/metabolism*; Kinetics; Mutation/genetics; Rec A Recombinases/genetics; Rec A Recombinases/metabolism*; SOS Response (Genetics); Templates, Genetic; Transcriptional Activation

Back
to Top