Title: Distinct pathways of homologous recombination controlled by the SWS1-SWSAP1-SPIDR complex.

Authors: Prakash, Rohit; Sandoval, Thomas; Morati, Florian; Zagelbaum, Jennifer A; Lim, Pei-Xin; White, Travis; Taylor, Brett; Wang, Raymond; Desclos, Emilie C B; Sullivan, Meghan R; Rein, Hayley L; Bernstein, Kara A; Krawczyk, Przemek M; Gautier, Jean; Modesti, Mauro; Vanoli, Fabio; Jasin, Maria

Published In Nat Commun, (2021 Jul 12)

Abstract: Homology-directed repair (HDR), a critical DNA repair pathway in mammalian cells, is complex, leading to multiple outcomes with different impacts on genomic integrity. However, the factors that control these different outcomes are often not well understood. Here we show that SWS1-SWSAP1-SPIDR controls distinct types of HDR. Despite their requirement for stable assembly of RAD51 recombinase at DNA damage sites, these proteins are not essential for intra-chromosomal HDR, providing insight into why patients and mice with mutations are viable. However, SWS1-SWSAP1-SPIDR is critical for inter-homolog HDR, the first mitotic factor identified specifically for this function. Furthermore, SWS1-SWSAP1-SPIDR drives the high level of sister-chromatid exchange, promotes long-range loss of heterozygosity often involved with cancer initiation, and impels the poor growth of BLM helicase-deficient cells. The relevance of these genetic interactions is evident as SWSAP1 loss prolongs Blm-mutant embryo survival, suggesting a possible druggable target for the treatment of Bloom syndrome.

PubMed ID: 34253720

MeSH Terms: Animals; Bloom Syndrome/genetics; Bloom Syndrome/pathology; Cell Proliferation; DNA-Binding Proteins/metabolism*; HEK293 Cells; Homologous Recombination/genetics*; Humans; Meiosis; Mice; Mitosis; Mouse Embryonic Stem Cells/metabolism; Multiprotein Complexes/metabolism*; Mutation/genetics; Phenotype; Rad51 Recombinase/metabolism; Sister Chromatid Exchange; Survival Analysis