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Publication Detail

Title: Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically Stable Covalent Interstrand Cross-Link Between a Guanine Residue and an Abasic Site.

Authors: Nejad, Maryam Imani; Guo, Xu; Housh, Kurt; Nel, Christopher; Yang, Zhiyu; Price, Nathan E; Wang, Yinsheng; Gates, Kent S

Published In Methods Mol Biol, (2019)

Abstract: Methods for the preparation of DNA duplexes containing interstrand covalent cross-links may facilitate research in the fields of biochemistry, molecular biology, nanotechnology, and materials science. Here we report methods for the synthesis and isolation of DNA duplexes containing a site-specific, chemically stable, reduced covalent interstrand cross-link between a guanine residue and an abasic site. The method uses experimental techniques and equipment that are common in most biochemical laboratories and inexpensive, commercially available oligonucleotides and reagents.

PubMed ID: 31016701 Exiting the NIEHS site

MeSH Terms: Cross-Linking Reagents/metabolism*; Guanine/chemistry*; Nucleic Acid Heteroduplexes/chemistry*; Oligodeoxyribonucleotides/chemical synthesis*; Oligodeoxyribonucleotides/chemistry

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