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Title: A Quantitative Proteomic Approach for the Identification of DNA Guanine Quadruplex-Binding Proteins.

Authors: Gao, Zi; Williams, Preston; Li, Lin; Wang, Yinsheng

Published In J Proteome Res, (2021 11 05)

Abstract: DNA sequences of high guanine (G) content have the potential to form G quadruplex (G4) structures. A more complete understanding about the biological functions of G4 DNA requires the investigation about how these structures are recognized by proteins. Here, we conducted exhaustive quantitative proteomic experiments to profile the interaction proteomes of G4 structures by employing different sequences of G4 DNA derived from the human telomere and the promoters of c-MYC and c-KIT genes. Our results led to the identification of a number of candidate G4-interacting proteins, many of which were discovered here for the first time. These included three proteins that can bind to all three DNA G4 structures and 78 other proteins that can bind selectively to one or two of the three DNA G4 structure(s). We also validated that GRSF1 can bind directly and selectively toward G4 structure derived from the c-MYC promoter. Our quantitative proteomic screening also led to the identification of a number of candidate "antireader" proteins of G4 DNA. Together, we uncovered a number of cellular proteins that exhibit general and selective recognitions of G4 folding patterns, which underscore the complexity of G4 DNA in biology and the importance of understanding fully the G4-interaction proteome.

PubMed ID: 34570971 Exiting the NIEHS site

MeSH Terms: DNA-Binding Proteins*/genetics; DNA/genetics; G-Quadruplexes*; Genes, myc; Guanine*/metabolism; Humans; Poly(A)-Binding Proteins/genetics; Poly(A)-Binding Proteins/metabolism; Promoter Regions, Genetic; Proteomics; Proto-Oncogene Proteins c-kit

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