Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Initial characterization of a type I fatty acid synthase and polyketide synthase multienzyme complex NorS in the biosynthesis of aflatoxin B(1).

Authors: Watanabe, Coran M H; Townsend, Craig A

Published In Chem Biol, (2002 Sep)

Abstract: The biosynthesis of the potent environmental carcinogen aflatoxin B(1) is initiated by norsolorinic acid synthase (NorS), a complex of an iterative type I polyketide synthase and a specialized yeast-like pair of fatty acid synthases. NorS has been partially purified from Aspergillus parasiticus, has been found to have a mass of approximately 1.4 x 10(6) Da, and carries out the synthesis of norsolorinic acid in the presence of acetylCoA, malonylCoA, and NADPH where hexanoylCoA is not a free intermediate. The N-acetylcysteamine thioester of hexanoic acid can substitute for the catalytic functions of HexA/B to initiate norsolorinic acid synthesis by the complex in the presence of only malonylCoA. An alpha(2)beta(2)gamma(2) stoichiometry is proposed for NorS in keeping with its estimated mass and the observed dimeric or higher-order quarternary structures of PKS and FAS enzymes.

PubMed ID: 12323372 Exiting the NIEHS site

MeSH Terms: Aflatoxin B1/biosynthesis*; Aflatoxin B1/chemistry; Anthraquinones/chemistry; Anthraquinones/metabolism*; Aspergillus/enzymology; Aspergillus/genetics; Autoradiography; Caproates/chemistry; Caproates/metabolism; Chromatography, High Pressure Liquid/methods; Coenzyme A/metabolism; Electrophoresis, Polyacrylamide Gel; Fatty Acid Synthases/metabolism*; Multienzyme Complexes/metabolism*; NADP/metabolism; Sulfhydryl Compounds/chemistry; Sulfhydryl Compounds/metabolism

Back
to Top