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Title: Rapid estrogenic regulation of extracellular signal- regulated kinase 1/2 signaling in cerebellar granule cells involves a G protein- and protein kinase A-dependent mechanism and intracellular activation of protein phosphatase 2A.

Authors: Belcher, Scott M; Le, Hoa H; Spurling, Lynda; Wong, Jeremy K

Published In Endocrinology, (2005 Dec)

Abstract: In neonatal rat cerebellar neurons, 17beta-estradiol (E(2)) rapidly stimulates ERK1/2 phosphorylation through a membrane-associated receptor. Here the mechanism of rapid E(2)-induced ERK1/2 signaling in primary cultured granule cells was investigated in more detail. The results of these studies show that E(2) and ICI182,780, a steroidal antagonist of estrogen receptor transactivation, rapidly increased ERK signaling with a time course similar to the transient activation induced by epidermal growth factor (EGF). However, EGF receptor (EGFR) autophosphorylation was not increased by E(2), and blockade of EGFR tyrosine kinase activity did not abrogate the rapid actions of E(2). The involvement of Src-tyrosine kinase activity was demonstrated by detection of increased c-Src phosphorylation in response to E(2) and by blockade of E(2)-induced ERK1/2 activation by inhibition of Src-family tyrosine kinase activity. Inhibition of Galphai signaling or protein kinase A (PKA) activity blocked the ability of ICI182,780 to rapidly stimulate ERK signaling. Under those conditions, E(2) treatment induced a rapid and transient suppression of basal ERK1/2 phosphorylation. Protein phosphatase 2A (PP2A) activity was rapidly increased by E(2) but not by E(2) covalently linked to BSA. Rapid E(2)-induced increases in PP2A activity were insensitive to pertussis toxin. The presented evidence indicates that the rapid effects of estrogens on ERK signaling in cerebellar granule cells are induced through a novel G protein-coupled receptor mechanism that requires PKA and Src-kinase activity to link E(2) to the ERK/MAPK signaling module. Along with stimulating ERK signaling, E(2) rapidly activates PP2A via an independent signaling mechanism that may serve as a cell-specific regulator of signal duration.

PubMed ID: 16123167 Exiting the NIEHS site

MeSH Terms: Animals; Cells, Cultured; Cerebellum/cytology; Cerebellum/metabolism*; Cyclic AMP-Dependent Protein Kinases/physiology*; Enzyme Activation/physiology; Estradiol/pharmacology; Estrogens/physiology*; Extracellular Signal-Regulated MAP Kinases/metabolism*; Female; GTP-Binding Proteins/physiology*; Intracellular Membranes/enzymology; Male; Mitogen-Activated Protein Kinase 1/metabolism; Mitogen-Activated Protein Kinase 3/metabolism; Neurons/metabolism; Pertussis Toxin/pharmacology; Phosphoprotein Phosphatase/metabolism*; Rats; Rats, Sprague-Dawley; Research Support, N.I.H., Extramural; Signal Transduction/drug effects; Signal Transduction/physiology*; Time Factors

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Last Reviewed: October 02, 2024