Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Cannabidiol promotes adipogenesis of human and mouse mesenchymal stem cells via PPARγ by inducing lipogenesis but not lipolysis.

Authors: Chang, Richard C; Thangavelu, Chloe S; Joloya, Erika M; Kuo, Angela; Li, Zhuorui; Blumberg, Bruce

Published In Biochem Pharmacol, (2022 Mar)

Abstract: Cannabidiol (CBD) is a non-psychoactive phytocannabinoid found in the Cannabis sativa plant. Human exposure to CBD can be through recreational marijuana use, commercially available CBD-containing products, and medical treatments. Previous studies found that cannabidiol may activate the master regulator of adipogenesis, peroxisome proliferator activated receptor gamma (PPARγ). Here we investigated the effects of CBD on adipogenesis in human and mouse multipotent mesenchymal stromal stem cells (MSCs). We tested the effects of CBD on nuclear receptor activation and adipogenic potential to demonstrate the mechanism of CBD effects and employed the in vitro MSC differentiation models to assess adipogenic effects of CBD.Using transient transfection assays, we demonstrated that CBD activated mouse and human PPARγ, but not its heterodimeric partner, the retinoid 'X' receptor, RXR. Our results showed that CBD increased lipid accumulation and the expression of adipogenic genes in mouse and human MSCs in vitro. Adipogenic differentiation induced by CBD was significantly decreased by the PPARγ antagonist T0070907, supporting the hypothesis that CBD promoted differentiation via PPARγ. Taken together, our results indicate that in humans and in mice, CBD induced adipogenic differentiation in MSCs through a PPARγ-dependent mechanism.

PubMed ID: 35026188 Exiting the NIEHS site

MeSH Terms: Adipogenesis/drug effects*; Adipogenesis/physiology; Animals; Benzamides/pharmacology; Cannabidiol/pharmacology*; Cell Line, Transformed; Humans; Lipogenesis/drug effects*; Lipogenesis/physiology; Lipolysis/drug effects*; Lipolysis/physiology; Mesenchymal Stem Cells/drug effects*; Mesenchymal Stem Cells/metabolism; Mice; PPAR gamma/agonists*; PPAR gamma/antagonists & inhibitors; PPAR gamma/metabolism; Pyridines/pharmacology

Back
to Top