Title: Association between DNA variant sites in the apolipoprotein A5 gene and coronary heart disease in Chinese.

Authors: Liu, Hekun; Zhang, Sizhong; Lin, Jianyin; Li, Hai; Huang, Aimin; Xiao, Cuiying; Li, Xuefei; Su, Zhiguang; Wang, Chunting; Nebert, Daniel W; Zhou, Bing; Zheng, Keqin; Shi, Jiajun; Li, Guixin; Huang, Dejia

Published In Metabolism, (2005 May)

Abstract: The recently discovered apolipoprotein A5 ( APOA5 ) gene has been shown to be important in determining plasma triglyceride levels, a major cardiovascular disease risk factor. We searched for possible associations of the APOA5 gene polymorphisms S19W and -1131T>C with coronary heart disease (CHD) in a Chinese population. A total of 483 Chinese CHD patients and 502 control non-CHD subjects were genotyped by polymerase chain reaction-restriction fragment length polymorphism for these 2 single nucleotide polymorphisms. We found that the minor allele 19W was observed only in CHD patients and not in controls, with allelic frequencies of 0.047 and 0.000, respectively ( P < .000001), and the minor allele -1131C was significantly higher in CHD patients than in controls (0.391 vs 0.299, P < .0001). These results suggest that both the S19W and -1131T>C variations in the APOA5 gene are associated with the CHD and appear to be 2 genetic risk factors for CHD susceptibility in Chinese. Moreover, we found that triglyceride levels were significantly higher in -1131C carriers than in -1131T subjects of the control group and that high-density-lipoprotein cholesterol was decreased in -1131C carriers among CHD patients.

PubMed ID: 15877284

MeSH Terms: Apolipoprotein A-V; Apolipoproteins A; Apolipoproteins/genetics*; Asian Continental Ancestry Group/genetics*; Case-Control Studies; Cholesterol, HDL/blood; Coronary Disease/blood; Coronary Disease/genetics*; Cytosine; DNA/genetics*; Fasting/blood; Female; Genetic Variation*; Heterozygote; Humans; Male; Middle Aged; Polymorphism, Single Nucleotide; Serine; Thymine; Triglycerides/blood; Tryptophan